Information on the thesis

The thesis is devoted to research the impact of 2-chloro-3-hydroxy-1,4-naphthoquinone and newly synthesized amide derivatives – 2-chloro-3-(3-oxo-3-(piperidine-1-yl)propylamine)-1,4-naphthoquinone (FO-1), 2-chloro-3-(3-(morpholine-4-yl)-3-oxopropylamine)-1,4-naphthoquinone (FO-2) on the lipid profile, prooxidant-antioxidant homeostasis and functioning of ion-transporting enzyme systems: Na⁺, K⁺– and summary Ca²⁺, Mg²⁺-dependent ATPases of embryonic cells during early embryogenesis.

It was established that all investigated compounds at concentrations of 10^-3-10^-5 М exhibit embryotoxic effect causing delay and abnormalities of embryos and larvae development that indicate an ability of the studied naphthoquinone derivatives to penetrate perivitelline space and blastomeres plasma membrane. Quinone derivatives cause damage to integrity of the cytoplasm membrane, the release of intracellular components into incubation medium and, ultimately, embryos death.

The ultrastructure of loach embryos which were incubated in medium with derivatives of 1,4-naphthoquinone at concentration of 10^-5 M during the first hour of development (2 blastomeres) and at sixth hour of development (1024 blastomeres) was characterized by a change in the electron density of the hyaloplasm and by the disorganization of organelles, occurs damage of mitochondria and endoplasmic reticulum membranes. It should be noted that all investigated compounds at concentrations of 10^-5 M cause destruction of the embryo cells structure, which shows itself as a violation of the integrity of the membranes, damage to the mitochondria, loss of christ, and an increase in the number of lysosomes.

The results of studies on the Misgurnus fossilis L. embryos which developed in the presence of 2-chloro-3-hydroxy-1,4-naphthoquinone and amide derivatives FO-1 and FO-2 in the medium of incubation showed that these compounds influenced on the lipid profile of the embryos and lead to a decrease in lipid content at all stages of development. Conducted «in vivo» and in vitro studies of the action of 2-chloro-3-hydroxy-1,4-naphthoquinone and the amide derivatives of FO-1 and FO-2 on the Misgurnus fossilis L. embryos indicate that these compounds exhibit dose-dependent inhibitory effect on the activity Na⁺, K⁺-ATPase and summary Ca²⁺-ATPase of embryos cell membranes, influence on the TBARS content and lead to imbalance of antioxidant defense system. The activities of superoxide dismutase and catalase increas in a dose-dependent manner while the activity of glutathione peroxidase decrease occurs.

According to the results of a two-factor ANOVA, it was first established that 2-chloro-3-hydroxy-1,4-naphthoquinone and amide derivatives of FO-1 and FO-2 have a significant impact on the content of TBARS, the activity of antioxidant system enzymes (superoxide dismutase, catalase, glutathione peroxidase), Na⁺, K⁺ and total Ca²⁺, Mg²⁺-dependent ATPase.

Key words: lipid profile, prooxidant-antioxidant balance, 2-chloro-3-hydroxy-1,4-naphthoquinone, amide derivatives of 1,4-naphthoquinone, Na⁺, K⁺-ATPase, total Ca²⁺, Mg²⁺-dependent ATPases.