Respondent

Bodnarchuk Nataliya Oleksandrivna

Theme

Changes in biophysical and morphological parameters of loach embryos for actions of flurenizyd

Defence Date

06.07.2018

Annotation

Dissertation is dedicated to the research of the free-radical reactions (in vitro and in
vivo)  of  loach  embryos  for  actions  of  flurenizyd  –  preparation  of  antimicrobial,
antituberculousis,  antichlamydia,  immunomodulator,  antioxidant,  hepatoprotective,
antiinflammatory,  antiviral  action.  Influence  of  flurenizyd  is  studied  on  functioning  of
Na+, K+-АТPаse of loach embryos Misgurnus fossilis L. on the stages of development 2,
16, 64, 256 and 1024 blastomeres. Flurenizide in all studied concentrations (0.01; 0.05;
0.15;  1;  5;  15  mM)  caused  increase  in  content  of  lipid  peroxidation  primary  products
(hydroperoxides) during development of embryos but decrease on stage 256 blastomeres.
The article contains data on ultrastructural and morphological changes of embryos
of loach Misgurnus fossilis L., what developed in incubation environment, which contain
flurenizyd.  It  is  shown  that  an  antibiotic  is  in  the  concentrations  of  1;  5;  15  mМ
predetermines  the  high  death  rate  of  embryos  of  loach  already  on  the  first  time  of  his
action. It is set that flurenizyd causes structural violations of mitohondria and cytoplasmic
net, causes the increase of amount of lysosomes at the embryos of loach on the stage of
development of 2 blastomeres. A degree of expressed of these changes is dosedependent.
The obtained results extend understanding of the mechanisms of flurenisid action on
membrane  processes  of  embryonic  cells.  These  data  can  be  used  for  toxicological
evaluation of bioactive or pharmacological substances, and therefore have value for human
and veterinary medicine.
After a two-factor analysis of variance for the first time found that the formation of
primary products of lipid peroxidation (hydroperoxides) has a significant effect of loach
embryos.  However,  the  formation  of  secondary  products  of  lipid  peroxidation
approximately the same effect commit time and flurenizyd and unmeasured factors.
At  superoxidedismutase,  catalase,  glutathioneperoxidase  and  glutathione-S-
transferase  activity  of  loach  embryos  exert  a  powerful  influence  unmeasured  factors,
which  may  include  external  factors  in  which  development  occurs.  We  found  that  the
development is more pronounced effect on catalase activity.
Applying  cluster  analysis  was  first  found  to  be  under  64  blastomeres  for  all
experimental groups studied parameters were divided into 4 groups of similarity. Revealed
that the concentration of flurenizyd 0,05mM not have a significant impact on performance,
the value of which was at the level of control (at 2 blastomeres). At the stage of 256 and
1024 all blastomeres study group on indicators are divided into 5 groups of similarity that
indicates a change flurenizyd impact on those stages of loach embryos.
The research results are important for flurenizyd use in the treatment of pregnant
women  as  well  as  for  further  research  on  its  effects  on  reproductive  function.  The
conclusions  arising  from  the  research  results  can  be  used  in  the  formation  of  new
regulations related to pharmacology.

Keywords:  loach  embryos,  flurenizyd,  embryogenesis,  blastomeres,  lipid
peroxidation, antioxidant enzymes system, plasma membrane, Na+, K+-ATPase.

Dissertation File

Autosummary File