Information on the thesis

Thesis  for  PhD  degree  in  biological,  speciality  03.00.04  –  biochemistry.
— Ivan Franko National University of Lviv, Lviv, 2015.
The aim was to study the condition of the pancreas, oxidative processes and
composition  of  lipids  and  fatty  acids in  the  blood, liver  and skeletal  muscles  of
rabbits with acute L-Arginine-induced pancreatitis and its correction by fed linseed
oil.
Experiments  were  conducted  on  male  breed  Gray  giant  rabbits  weighing
3.8-4.0 kg. Animals were divided into five groups (five rabbits each): control (С);
control  animals  fed  linseed  oil  (С+linseed  oil);  experimental  animals  with
L-arginine-induced  acute  pancreatitis  (P);  animals  with  experimental  L-arginine-
induced  acute  pancreatitis,  fed  linseed  oil  (P+linseed  oil);  experimental  animals
with  L-arginine-induced  acute  pancreatitis,  fed  sunflower  oil  (P+sunflower  oil).
Rabbits  of  all  groups  were  fed  a  standard  granulated  feed  in  an  amount  of
225 g/head/day  during  one  month  and  drinking  water  without  restrictions.
However,  during  this  period  rabbits  of  С+linseed  oil  and  P+linseed  oil  groups
received feed with daily inflicted on him linseed oil and rabbits of P + sunflower
oil group received feed with daily inflicted on him sunflower oil per 1 ml/kg body
weight. In addition five days before the end of the experiment, rabbits  of C and
С+linseed  oil  groups  once  received  an  intraperitoneal  injection  of  2  ml/kg
physiological  sodium  chloride  solution,  and  rabbits  of  P,  P+linseed  oil  and
P+sunflower oil groups  in the same amount of saline received L-arginine at a dose
of 4 g/kg body weight. At the end of the experiment the rabbits were slaughtered
by  decapitation  under  ether  anesthesia.  Samples  of  blood,  pancreas,  liver  and
skeletal muscles were studied.
It  was  established  that  the  activity  of  α-amylase  and  lipase  in  the  blood
plasma and glutathione peroxidase and superoxide dismutase in erythrocytes, liver
and skeletal muscles, the number of necrotic acinar epithelial cells in the head and
tail of the pancreas and the content of diene conjugates of hydroperoxides lipids
and malondialdehyde, unesterified and esterified cholesterol increased in the blood
plasma,  liver  and  skeletal  muscles  of  rabbits  with  acute  L-Arginine-induced
pancreatitis  and  amplified  of  rabbits  with  acute  L-Arginine-induced  pancreatitis
feeding  sunflower  oil,  which  is  a  source  of  proinflammatory  linoleic  acid,  and
normalized of rabbits with acute L-arginine-induced pancreatitis corrected by fed
linseed oil, that contains in its composition a large number of  anti-inflammatory
linolenic acid. Besides, catalase activity in erythrocytes, liver and skeletal muscles
and concentration of nonetherified   fatty acids reduced in the blood plasma, liver
and  skeletal  muscles  of  rabbits  with  acute  L-Arginine-induced  pancreatitis  and
sharply  decreased  of  rabbits  with  acute  L-Arginine-induced  pancreatitis  feeding
sunflower  oil,  and  normalized  of  rabbits  with  acute  L-Arginine-induced
pancreatitis corrected by fed linseed oil. At the same time, the ratio of сontent of
anti-inflammatory polyunsaturated fatty acids (omega-3) to pro-inflammatory fatty
acids  (omega-6)  of  total  lipids,  phospholipids,  esterified  cholesterol  and
triacylglycerol  was  reduced  in  the  blood  plasma,  liver  and  skeletal  muscles  of
rabbits with acute L-Arginine-induced pancreatitis and was markedly reduced in
rabbits with acute L-Arginine-induced pancreatitis fed with sunflower oil. It was
increased  in  rabbits  with  acute  L-Arginine-induced  pancreatitis  corrected  by  fed
linseed  oil.  Besides,  the  ratio  of  nonetherified   anti-inflammatory  fatty  acids
omega-3  to nonetherified   inflammatory  fatty  acids  omega-6 greatly  increased  in
mentioned   tissues  of  rabbits  with  acute  L-Arginine-induced  pancreatitis  feeding
linseed oil.
The content of derivatives of cholesterol – bile acids was reduced in blood of
rabbits with acute L-Arginine-induced pancreatitis. The concentration of bile acids,
25-OH vitamin D 3 , testosterone, cortisol and aldosterone was sharply decreases in
blood of rabbits with acute L-Arginine-induced pancreatitis fed with sunflower oil,
and increased in rabbits with acute L-Arginine-induced pancreatitis corrected by
fed linseed oil.
Keywords: rabbits, acute arginine pancreatitis, correction, lipids, fatty acids,
derivatives of cholesterol.