Respondent

Khrabko Maryana Ivanivna

Theme

Immunophysiological, reproductive and detoxification functions of the rats organism under the action of germanium citrate

Defence Date

19.04.2019

Annotation

The dissertation is devoted to the research of the influence of various doses of germanium
citrate obtained by nanotechnology methods and chemical synthesis, on the activity of immune,
antioxidant, detoxification and reproductive systems of the rats organism of different generations;
clarification of immune-physiological influence of various germanium citrate doses;
establishment of age, reproductive and systemic features of their influence at long-term
application during pregnancy, lactation, breastfeeding period, physiological and reproductive
maturation, embryonic and fetal development. The stimulating effect of low and average doses of
nanogermanium citrate, obtained by the nanotechnology method was established on immune
physiological and hematological parameters of the organism, and also on inhibiting – at high dose
application. Chemically synthesized germanium citrate has an inhibitory effect at action of
200 µg Ge on the content of immune globulins, glycoproteins and their carbohydrate components
in the blood of F 0 rats, as well as the growth and development of the organism and individual
organs in F 1 females. The regulatory influence of the applied doses of germanium citrate on the
ontogenetic development of young rats in two generations and less expressed on the internal
organs indices according to the coefficients of the heart, liver, kidneys, testicular, lungs, and
spleen masses were proved. For the first time new data was obtained on the histological structure
of the immune system organs of rats at the long-term influence of various doses of germanium
citrate, and the connection of changes in physiological and biochemical parameters of blood and
tissues with morphological and structural features of these organs structure was noted. The dose-
dependent influence of germanium citrate on the reproductive ability of rat females of F 0 and F 1
generations, resistance, viability and ontogenetic development of their young rats during 2-
4.5 months of life was proved. Based on the determination of Ca, P, Fe, Cu, Zn, Mn, Co and Ge
content in rat tissues, the regulatory influence of germanium citrate in the applied doses on their
distribution in tissues and organs of rats dependence of the content of these elements on the dose
and method of synthesis of germanium citrate was determined.
The application of different germanium citrate doses in females of two generations of
rats caused regulatory influence on the immune physiological and antioxidant state of the
organism. Influence of germanium citrate in F 0 female rats was characterized by a decrease in
blood aminotransferase activity, concentration of Ca, P, triacylglycerols, creatinine – at action
of 200 and 2000 µg, with an increase at the action of 10 and 20 µg Ge, as well as the content of
phenolsulphates and phenolglucuronides in liver, kidney and muscles at action of 20 µg Ge. In
the blood of F 1 females, a significantly lower Ca content at action of 10, 20 and 200 µg Ge; and
albumin and triacylglycerols was detected – at the action of 2000 µg Ge against the background
of higher P content in animals receiving 10 and 20 µg of Ge.
Application of germanium citrate increased the multiplicity of F 0 female rats fertility at
action of 20, 200 and 2 000 µg Ge (P <0.001), but the preservation of young rats at 20 and 40th
days was lower in comparison to control in all experimental groups, with the lowest values at
the action of 2000 µg Ge. In F 1 females, a decrease in preimplantation was observed, but an
increase in post-implantation and total embryonic lethality at action of 200 and 2,000 µg Ge,
which resulted in a decrease in the number of live fetuses in females receiving 2000 µg Ge.
The application of germanium citrate to F 0 females during pregnancy and lactation
stimulated the growth and development of F 1 young rats in the first 20 days of life at action of
10 and 20 µg of Ge. Adding germanium citrate to F 1 rats in subsequent periods of ontogenesis
provided higher in comparison to control intensity of their growth by 5.4; 12.2 and 5.0 %,
respectively, on the 120 day of life at the action of 10, 20 and 200 µg Ge, but lower by 8.9 % –
at the action of 2000 µg Ge. The stimulating effect of germination citrate in a dose of 20 µg on
the development of embryos and fetuses in F 1 females and inhibiting – in a dose of 200 µg of
Ge was established.
Application of different doses of germanium citrate resulted in a corrective effect on the
state of the immune system of the F 1 male organism, with an increase in the content of medium
molecules in the blood at the age of 2-2.5 months. At the age of 4-4.5 months, higher blood
levels of immune globulins, sialic acids, and medium-molecules, but lower, in circulating
immune complexes at action of 10, 20 and 2000 µg Ge was established.
Key words: physiological state, organism development, resistance, hematological
parameters, doses of germanium, mineral metabolism.

Dissertation File

Autosummary File