GENETIC INSTABILITY OF STREPTOMYCES AMBOFACIENS RESISTANCE
TO CHLORAMPHENICOL
V. Fedorenko
Ivan Franko National University of Lviv, Hrushevsky³ Sr. 4,
UA-79005 Lviv, Ukraine. e-mail: genetic@franko.lviv.ua
The resistance of spiramycin producer, S.ambofaciens to chloramphenicol is an unstable trait. Spontaneous mutants sensitive to chloramphenicol (Cmls) arise in the culture of S.ambofaciens with high frequency (ca. 10-3) and after step-by-step selection selection it produces spontaneous mutants with raised resistance to chloramphenicol (Cmlr). The corelation between Cmlr / Cmls phenotypes and resistance / sensitivity to macrolides and lincomycin was shown. Cmlr - mutants are characterised by lower variability of antibiotic activity level comparable to parental strains of S.ambofaciens. Amplification of nucleotide sequences were not found in total DNA of S.ambofacienas 1115 and it's Cmlr and Cmls - mutants in contrary to another wilde type strain S.ambofaciens 792 and its antibitic resistant mutants.
Key words: Streptomyces, antibiotics, chloramphenicol, resistance.
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