INVESTIGATION OF ACTIVITY OF RIBOFLAVIN KINASE - THE FIRST
STEP ENZYME IN FLAVIN NUCLEOTIDES BIOSYNTHESIS IN
MICROORGANISMS
I.Bilinskaya, Z.Gavrylenko, V.Kashchenko
Ivan Franco National University of Lviv,Hrushevskyi St..4, UA-79005 Lviv,Ukraine.
e-mail: biolog@.franco.Lviv.ua
The activity of riboflavin kinase (ATP : riboflavin 5'-phosphotransferase, E.C. 2.7.1.26), the first step enzyme in flavin nucleotides biosynthesis was investigated in 57 strains of microorganisms belonging to different systematic groups: bacteria, streptomycetes, yeasts and filamentous fungi. It was shown that bacteria exhibit low riboflavin kinase activity and enzyme's activity with riboflavin or 1,5 - dihydroriboflavin as substrates did not differ significantly. High riboflavin kinase activity was observed in methanol- and n-alcane-utilizing yeasts, as well as in filamentous fungi which are industrial oversynthetics of riboflavin and FAD.
Key words: riboflavin, 1,5-dihydroriboflavin, riboflavin kinase, bacteria, yeasts,fungi.
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