THE INFLUENCE OF CAFFEINE ON GENETIC INSTABILITY IN
STTREPTOMYCES COELICOLOR A3(2)
O.Maryina, S.Zavorotna, V.Fedorenko
Ivan Franco National University of Lviv, Hrushevskyi St. 4, UA-79005 Lviv, Ukraine
Spontaneous mutants of S.coelicolor A3(2) sensitive to chloramphenicol (CMS) arise at frequency of 0,3%. The ultraviolet irradiation (UV) of S.coelicolor A3(2) spores causes the elevation of CMS-mutants frequency to 37%. 52% of these mutants were unstable and produced CMS and CMR derivatives in next generation. The presence of subinhibitory concentration of caffeine in medium increases the lethal effect of UV - irradiation and lowers the fraction of UV - induced CMS clones to 12%. Only 15% of CMS-mutants obtained on the medium with caffeine were unstable. Probably, the caffeine sensitive repair system is involved in cml genes instability in S.coelicolor A3(2).
Key words: Streptomyces, genetic instability, caffeine.
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