CHOLINERGIC MECHANISM OF REGULATION IN ADDAPTIVE REACTIONS
OF TREATED IONIZING RADIATION ORGANISM AND THEIR CORRECTION
BY EXOGENOUSE SUBSTRATES OF KREBS CYCLE
N.Kurgalyuk, O.Goryn, O.Ikkert, S.Gordii
Ivan Franco National University of Lviv, Hrushevskyi St.4, UA-79005 Lviv, Ukraine
e-mail: biolog @ franko.lviv.ua
We have study the effect of exogenouse substrates of Krebs cycle ( sodium alpha-ketoglutarate (KG), malate, succinate, glutamate) on ADP-stimulation respiration of mitochondria from hepar of rats under the. ionizing radiation treatment. It has been shown that 30-days (10, 20 and 30 Roentgen in total) results in activation of endogenouse succinic acid oxidation. Exogenous alpha-ketoglutarate (KG) concentration causes increase in Mch KG and decrease in syccinic acid oxidation of treated organisms. This changes in metabolite providing of Krebs cycle enzymes support intensification of reparation treated structures and increase of organism`s cholinergic resistance. It provides intensive support of Krebs cycle substrates and in increase of living activity of organisms in extremal conditions.Intraperitoneal KG injection in course doses have been made 10 days after daily dose 1 Roentgen treated decreases the inhibition of respiration and oxidative phosphorylation, increases the efficiency of respiration during the period from 1-st injected till to end of experiment.
Key words: ionizing radiation, substrates of Krebs cycle, rat liver mitochondria, mitochondrial respiration, oxidative phosphorilation.
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