BIOCHEMICAL AND MORPHOLOGICAL REACTIONS OF ORGANISMS
POLYRESISTANT TUBERCULOSIS TREATMENT IN EXPERIMENT
K. Mazhak, D. Sanagurskiy, I. Oliynyk
Ivan Franko National University of Lviv, Hrushevskyi st.4,UA-79005 Lviv, Ukraine
e-mail: biolog@ franco.Lviv.ua
In 120 guinea pigs, 100 of which were infected with virulent culture of tuberculosis microbacteria in dosage 0,01 mg per 1 kg of mass in organs (liver, lungs) and cellular structures, were studied the peculiarities of changes in activity of lactate and malate dehydrogenases and their isoforms on various development stages of generalized and fibrinocavemous processes with spontaneous proceeding as well as during treatment with new antituberculosis preparations Rifabutin and Phlurenisid. There was investigated the influence of natrium succinate. The efficiency of treatment were estimated by the data of macroscopic, histological and bacteriological researches.
There was determined that tuberculosis infection was developed and progressed on the background of expressed changes from the site of investigated ferments, disorders of structure and functions of organs irrespective of infection locus. The detected disorders have undulatory character and differ in their depth and direction in heterospecialized organs.
Application of one kind of means, even the most effective (Rifabutin + Phlurenizid) tuberculostatic means do not result in complete release from bacteria and healing of destruction focus, and sometimes even creates non-favorable influence on proceeding of metabolic processes. Natrium succinate in dosage 200 mg/kg of mass, combined with Rifabutin and Phlurenizid considerably accelerates the therapeutic aspect, making it more integrated and promoting normalization of investigated processes.
Key words: lactate dehydrogenase, malate dehydrogenase, tuberculos, tuberculosis microbacteria.
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