CLONING AND SEQUENCING OF A PUTATIVE POSITIVE TRANSCRIPTION REGULATOR GENE
OF LANDOMYCIN E BIOSYNTHETIC GENE CLUSTER OF STREPTOMYCES GLOBISPORUS 1912
K. Pankevych*, H. Kruegel**, V. Fedorenko*
*Ivan Franko National University of L'viv,
Hrushevskoho st. 4, L'viv 79005, Ukraine
**Hans-Knoell-Institut for Natural Product Research,
Beutenbergstrasse, 11, Jena 07745, BRD
The DNA fragment of 1515 bp from landomycin E biosynthetic gene cluster of Streptomyces globisporus 1912 was sequenced. Within this fragment an open reading frame of 783 bp was identified. Computer assisted comparative analysis of its putative translational product (291 aa) revealed high homology with the large family of transcription regulator proteins of bacterial two-component sensory transduction systems. The putative gene contained an unusual for GC-rich streptomycete DNA codon TTA, that was found only in antibiotic resistance genes and regulatory genes of secondary metabolism. This data suggested that we cloned a putative positive transcription regulator gene lndI, the protein product of which - LndI - could induce expression of landomycin E biosynthetic genes in Streptomyces globisporus 1912.
Key words: sensory transduction, Streptomyces, transcription regulation, two-component system.
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