STREPTOMYCES GLOBISPORUS 1912 MUTANTS WITH ALTERED LANDOMYCIN E BIOSYNTHESIS
B. Ostash, L. Basiliya, O. Gromyko, Yu. Rebets, V. Fedorenko
Ivan Franko National University of L'viv,
Hrushevskogo st. 4, L'viv 79005, Ukraine,
e-mail: genetic@franko.lviv.ua
Ten Streptomyces globisporus stable mutants with interrupted or ceased synthesis of novel angucycline antibiotic landomycin E (LE) were examined by thin layer chromatography, spectrophotometry, and Southern hybridization. Four strains (LND23, LND29, LND81, LND901) with completely abolished LE production and three strains (LND317,LND801, LND900) with altered were identified. Strains LND23, LND900, LND901, W1 carry deletions within lnd-cluster. In case of strain W1, we did not observe hybridization of W1 total DNA restricts with all DNA-probes used; LND900 and LND901 strains carry deletion of M-fragment (lndI gene, possible transcriptional activator of lnd-genes) of lnd -cluster, LND23 - deletions of M- and N-fragments (genes: lndI; lndE- putative oxygenase, lndA, B - minimal polyketide synthase).
Key words: landomycin E, Streptomyces, angucyclines, mutants.
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