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STUDY OF EXPRESSION NUCLEASES IN CELLS OF
MAMMALS BY METHOD OF PROTEIN RENATURATION IN
POLYAKRYLAMIDE GEL
Yu.Kit, G.Shuvayeva, V.Drel’, N.Igumentseva, L.Drobot
Institute of Cell Biology, National Academy of Sciences of Ukraine,
Dragomanov st. 14/16, L’viv 79005, Ukraine,
e-mail: kit@biochem.lviv.ua
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The method of analysis of enzymatic activity in polyacrylamide
gel bases on high performance separation of proteins SDS-electrophoresis with
their subsequent renaturation and detection of enzymatic activity. It was used
for analysis of an expression of nucleases in cells in culture of lines HEK293;
NIH/3T3; U937. It was found that in HEK293 cells were expressed nucleases with
molecular weights 47 and 45 kDa. The localization of these nucleases in cell
nuclei was shown as well. It was found, that the fractions of histones after
denaturation are catalyzed of DNA degradation.
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Keywords: Culture of cells, nuclei, nucleases, electrophoresis.
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