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THE ROLE OF ANTIOXIDANTS IN X-RAYS INDUCED
MUTAGENESIS IN DROSOPHILA MELANOGASTER
N.Kimak-Holub, M.Smyk, Ya.Chernyk
Ivan Franko National University of L'viv,
Hrushevskoho st.4, L'viv 79005, Ukraine,
e-mail: herbarium@franko.lviv.ua
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The influence of different doses of antioxidants (AO) –
a-tocopherol and b- carotene-on survival and the frequency of dominant lethal
mutations (DLM) appearance and visible mutations in X-chromosome of
D.melanogaster has been investigated; the role of these AO in X-rays induced
mutagenesis has been analyzed. It has been shown that toxical effect of
a-tocopherol and b-carotene in certain concentrations reveals on different
stages of early ontogeny. By the method of DLM it has been testified that the
embriogeny stage is the most sensitive to the AO influence. When a-tocopherol
has been added to the fly food before irradiation we observed decreasing of
frequency of Xchromosome mutants appearance since F2 comparing to the frequency
obtained after 3000 R action. This was an evidence that this antioxidant
(a-tocopherol) had prolonged radioprotective effect.
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Keywords: Drosophila,irradiation, antioxidants, mutagenesis, DLM.
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