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INFLUENCE OF ARS-FLANKING SEQUENCES ON STABILITY
OF PICHIA GUILLIERMONDII/ ESCHERICHIA COLI SHUTTLE
PLASMIDS IN THE YEAST CELLS
Î. Khalimonchuk, À. Petryshyn, Y. Pynyaha, D. Fedorovych, À. Dorosh, Y.
Boretsky
Institute of Cell Biology, National Academy of Sciences of Ukraine,
Dragomanova st. 14/16, L’viv 79005, Ukraine,
e-mail: okhalimonchuck@yahoo.com
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Pichia guilliermondii ARS – element was subcloned on pUC19 and
modified pBR322 vectors as a part of 0,82kb HincII fragment of chromosomal DNA.
Several plasmids, based on these newly obtained vectors were constructed.
Plasmid stability dependence on length of native ARS-flanking sequences was
studied. Increase of size of ARS carrying sequences rises plasmid stability in
4–6 fold. Thus, length of native ARS-flanking sequences is important for
plasmid stability in P. guilliermondii cells.
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Keywords: yeast, Pichia guilliermondii, ARS flanking sequences, plasmid
stability.
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