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IDENTIFICATION OF Ñà2+-ÀCTIVATED, Ìg2+-DEPENDENT
ÀÒÔ-ase IN MEMBRANE MICROSOMAL FRACTION OF
SECRETORY CELL OF ISOLATED ACINI FROM RAT
SUBMANDIBULAR SALIVARY GLAND
N. Fedirko, Ju. Vats, M. Klevets
Ivan Franko National University of L’viv,
Hrushevsoho st. 4, L’viv 79005, Ukraine,
e-mail: biolog@franko.lviv.ua
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The method for investigation the ATPase-systems activities of
membrane microsomal fraction of isolated acini from rat submandibular salivary
gland has been elaborated. It was revealed Mg2+-ATÔ-ase, Ñà2+, Mg2+-ATÔ-ase and
Na+, K+-ÀÒÔ-ase activities in the membrane vesicles fraction of secretory
cells. Speci fically, Ñà2+, Mg2+-ATÔ- ase activity has been shown in the plasma
membrane (2,74 nmol г/mg protein per 1 hour) and endoplasmic membrane (2,66
nmol г/mg protein per 1 hour) vesicles. Additionally using dygitonin treatment
of membrane microsomal fraction we have shown that mostly investigated fraction
contains inside-out vesicles.
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Keywords: secretion, plasma membrane, Ñà2+, Mg2+-ATÔ-ase, dygitonin.
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