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INVESTIGATION OF DIFFERENT LIGAND FORMS OF
GEMOGLOBIN BY THE METHOD OF ELECTRONIC
SPECTROSCOPY
K. Dudok, R. Bilyi, A. Fedorovych
Ivan Franko National University of L’viv,
Hrushevskoho st., 4, L’viv 79005, Ukraine.
e-mail: rbilyi@yahoo.com
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Spectrophotometrical characteristics of deoxy-, oxy-, carboxy- and methemoglobin
were investigated. Energy calculations of electron transitions were carried out. In deoxyhemoglobin
not only ion Fe(II) but also Nitrogen atoms move out of porphyrin plain. In
oxyhemoglobin bond formation between Fe(II) dxz–orbitals and π-orbitals of O2. Calculated
energy of electron transitions in methemoglobin is rather small. This suggest the
π→π* electron transition which take place when ion-radical complex of porphyrin ring
and oxydized form of Fe(III) is formed.
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Keywords: oxyhemoglobin, deoxyhemoglobin, electron transitions.
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