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INDUCTION OF DOMINANT LETHAL MUTATIONS AND XLINKED
VISIBLE MUTATIONS BY NITROSOETHYL UREA IN
DROSOPHILA MELANOGASTER
N. Holub, I. Kozarevska, Ya. Chernyk
Ivan Franko National University of L’viv,
Hrushevskoho st. 4, L’viv 79005, Ukraine,
e-mail: holub_74@yahoo.com
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The influence of nitrosoethylurea (ENU) on induction of dominant lethal mutations
(DLM), appearance of visible mutants and mutants with changes in brain structures linked
to X-chromosome of Drosophila melanogaster have been investigated. It was shown
that ENU in dose of 0,5 mkg/ml (LD50 for laboratory strain
y2wa4) leads to 6 times increasing
the frequency of DLM appearance comparing with control. This concentration of
ENU causes the appearance of visible mutants in X-chromosome with 2,6x10-4-4,3x10-4
frequency within two generations. In concentration 0,4 mkg/ml mutagene induces the
high frequency of changes in brain structures of Drosophila which appeare in different
periods of imago life (in the age of 5, 10 and 30 days). All mutants cultures with neurodegenerative
brain changes are characterized by flies rapid dying off and reduced indexes
of average life span.
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Keywords: drosophila, nitrosoethylurea, DLM, mutagenesis, neurodegeneration.
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