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A GENE CLONING SYSTEM FOR STREPTOMYCES
CYANOGENUS S136
A. Luzhetskyy*, M. Fedoryshyn*, D. Hoffmeister**, A. Bechthold**,
V. Fedorenko*
*Ivan Franko National University of L’viv,
Hrushevskoho st. 4, L’viv 79005, Ukraine,
e-mail: genetic@franko.lviv.ua,
**Albert-Ludwigs-University Freiburg, Pharmazeutische Biologie,
Stefan-Meier-Strasse 19, Freiburg D-79104, Germany,
e-mail: bechthold@uni-freiburg.de
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To introduce DNA into Streptomyces cyanogenus S136, we explored an intergeneric
conjugation method. Plasmids pSET152, pSOK101, pSOK201, and pCHZ101 were
transferred from Escherichia coli ET12567(pUB307) with different frequencies. The status
of plasmids in the recipient strain and the stability of their inheritance were investigated.
Hybridization analyses of several exconjugants indicated that pSET152 inserted into
two attB sites on the chromosome. The insertion of pSET152 had no effect on landomycin
A production. pSET152 was stably inherited even under non-selective conditions. A
series of non-replicative plasmids harboring different insertions from the landomycin gene
cluster were constructed to study the efficiency of homologous recombination in S. cyanogenus S136.
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Keywords: streptomyces, angucycline antibiotics, conjugation.
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